Kinase B

Kuriyan Lab

2D 1H - 15N TROSY spectrum of a 300 residue kinase acquired on the Bay Area 900 instrument before installation of the cryoprobe. The spectrum was recorded on a 15N, 13C, and 2H-labeled sample at a concentration of approximately 750 uM in a 5 mm NMR tube. Collection of the spectrum at 900 MHz was critial to resolve many of the resonances.

2D 1H - 13C projection of a 3D TROSY-HNCA spectrum of the kinase. The spectrum was recorded on a sample similar to that used for the 2D TROSY spectrum.