Kinase A

Kuriyan Lab

2D 1H-15N TROSY spectrum of a 270 residue kinase acquired at 900 MHz. The sample was 15N,13C, and 2H-labeled and at a concentration of 300 uM.

2D 1H - 13C projection of a 3D TROSY-HNCA spectrum of the kinase.  Many 13Calpha and 13Calpha(i-1) signals were visible.  Notably, a similar spectrum recorded on a Bruker 800 MHz system with a  cryoprobe yielded more signals. The reduced number of signals apparent at 900 MHz maybe due to broadening associated with moderate-scale (ms) dynamics.