2D 1H-15N HSQC spectrum of the same 35 kDa tumor suppressor protein at 600 MHz. The sample was 15N-labeled, but not deuteriated. At lower field and without deuteriation, the sample quality is much worse. The combination of deuteriation and availability of the 900 MHz instrument makes this project possible.
2D 1H-15N TROSY spectrum of a 35 kDa tumor suppressor protein at 900 MHz. The sample was both 15N- and 2H-labeled.