Research Description.

I am working on the development of an ultrahigh-throughput microdroplet generator array system for co-encapsulating single cell and single primer-functionalized bead in each uniform nanolitre-sized droplet for single-cell genetic analysis (SCGA). I am also working with Richard Novak to implement this SCGA system in detecting low-frequency mutations and polymorphisms in cell populations for cancer diagnosis, such as the t(14:18) Bcl2-İgH translocation in the RL lymphoma cell line.

References and Publications

1. Yong Zeng, Mei He, D. J. Harrison, “Large-Scale Microfluidic Patterning of Colloidal Nanoarrays for High-Throughput Continuous DNA Fractionation”, Angew. Chem. Int. Ed.  2008, 47, 6388-6391.
2. Mei He, Yong Zeng, Xuejun Sun, D. J. Harrison, “Confinement effects on the morphology of photopatterned porous polymer monoliths for capillary and microchip electrophoresis of proteins”, Electrophoresis, 2008, 29, 2980-2986.
3. Yong Zeng, D. J. Harrison, “Self-Assembled Colloidal Arrays as Three-Dimensional Nanofluidic Sieves for Separation of Biomolecules on Microchips”, Anal. Chem., 2007, 79, 2289-2295. (Highlighted in the Research Profile: “Nanofluidic Sieves Ease Separation Anxiety”, Anal. Chem., 2007, 79, 2611)
4. Yong Zeng, D. J. Harrison, “Confinement Effects on Electromigration of Long DNA Molecules in an Ordered Cavity Array”, Electrophoresis, 2006, 27, 3747-3752.
5. Yong Zeng, H. Chen, D. W. Pang, Z. L. Wang, J. K. Cheng, “Microchip Capillary Electrophoresis with Electrochemical Detection”, Anal. Chem., 2002, 74, 2441-2445.
6. H. Chen, Yong Zeng, B. F. Liu, D. W. Pang, J. K. Cheng, “Influence of Soluble Polymer Polyvinylpyrrolidone on Separation of Small Peptides and Amino Acids by Microchip-Based Capillary Electrophoresis”, Anal. Bioanal. Chem., 2002, 373, 314-317.

Conference Presentations

• Yong Zeng, P. Kumaresan, N.M. Toriello, E.S. Douglas, C.J. Yang, R.G. Blazej, S.A. Cronier, N. Thaitrong. S.C.H. Hsaio, M.B. Francis, C.R. Bertozzi, and R.A. Mathies, “Microfluidic Technologies for Single Cell Genetic and Expression Analysis” 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2007), San Diego, CA, USA, Oct. 2008. (Poster)
• Neda Nazemifard, Yong Zeng, D.J. Harrison, S. Bhattacharjee, J. Masliyah, “DNA Transport and Fractionation through a Self-Assembled Nanoparticle Array in Presence of Pulsed Electric Field” The 82nd ACS Colloid & Surface Science Symposium, Raleigh, NC, USA, June 2008. (Oral)
• Yong Zeng, D. J. Harrison, “High-Throughput DNA Microfractionator Using Self-Patterned Large-Scale Crystalline Nanoarrays”, 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2007), Paris, France, Oct. 2007. (Oral)
• Mei He, Yong Zeng, D. J. Harrison, “Confinement Effect on The Structure of Polymer Monoliths Photopatterned within Microchannels”, 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2007), Paris, France, Oct. 2007. (Poster)
• Yong Zeng, D. J. Harrison, “On-Chip Biomolecule Separation Using Self-Assembled Colloidal Nanoarrays”, MSB 2007: 21th International Symposium on Microscale Bioseparations, Vancouver, BC, Canada, Jan. 2007. (Oral)
• Yong Zeng, D. J. Harrison, “Self-Assembled Three-Dimensional Nanofluidic Sieves for Bioseparation”, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2006), Tokyo, Japan, Nov. 2006, pp. 1020-1022. (Oral)
• Yong Zeng, D. J. Harrison, “Ordered Nanopore Cavity Array Structured by Colloidal Templating for Electrophoresis of Large DNA Molecules”, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2005), Boston, MA, USA, Oct. 2005, pp. 494-496. (Oral)
• Yong Zeng, D. J. Harrison, “Electrophoresis of Single DNA Molecules in an Ordered Nanopore Network Fabricated by Colloidal Templating”, 88th Canadian Chemistry Conference and Exhibition, Saskatoon, SK, Canada, May 2005. (Oral)