Ph.D. Candidate, Chemical Engineering
B.S. in Chemical Engineering from UCLA
I am a sixth year graduate student from the Chemical Engineering department. I am co-advised by Professor Richard Mathies in the Chemistry department and Professor Roya Maboudian in the Chemical Engineering department. My current project is focused on developing a 96 PCR-CE (Polymerase Chain Reaction- Capillary Electrophoresis) micro-plate for more efficient and massively parallel DNA analysis.
The advantages of performing
PCR and CE on a micro-plate over their conventional counterparts have
already been shown in the works of Eric Lagally and Brian Paegel in our
group. With the successful demonstration of a single integrated PCR-CE
device by Eric, my first task is to improve his current vent/valve system
to develop an easy-to-fabricate and a highly parallel vent/valve system
to achieve a 96 PCR-CE device on a chip. To date, I have successfully
developed a PDMS membrane valve, which met the criteria mentioned above.
Next step is to investigate the different combinations vent/valve systems
for the PCR reaction chamber.
Figure 1:
Top left: 3-D schematic of
a PDMS membrane valve
Bottom left: Cross-sectional schematic of a PDMS membrane valve
Top right: Breakdown pressure of the PDMS membrane valve
Bottom right: Flow characteristic of the PDMS membrane valve
Figure 2: Schematic of a 96 Sample PCR-CE Microprocessor
References and Publications
William H. Grover, Alison M. Skelley, Chung N.
Liu, Eric T. Lagally and Richard A. Mathies. Monolithic membrane valves
and diaphragm pumps for practical large-scale integration into glass microfluidic
devices. Sensors and Actuators B, 89 (3), 315-323,
(2003).
[Full
Text in HTML]
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William H. Grover, Alison M. Skelley, Chung N. Liu, Eric T. Lagally and Richard A. Mathies. Practical valves and pumps for large-scale integration into microfluidic analysis devices. Proceedings of the Sixth International Symposium on Micro Total Analysis Systems, Nara, Japan, November 3-7, 2002.
Richard A. Mathies, Eric T. Lagally, Toshihiro Kamei, William H. Grover, Chung N. Liu and James R. Scherer. Capillary array electrophoresis bioprocessors. Proceedings of the Solid-State Sensor, Actuator and Microsystems Workshop, Hilton Head, South Carolina, June 2-6, 2002, p. 112-117.
Paegel, B.
M., Emrich, C. A., Wedemayer, G. J., Scherer, J. R., and Mathies, R. A.
High-Throughput DNA Sequencing with a Microfabricated 96-Lane Capillary
Array Electrophoresis Bioprocessor, Proc. Natl. Acad. Sci. U. S. A.,
99: 574-579 (2002).
[Abstract]
[Full Text in
HTML] [Full Text in PDF]
Lagally, E.T.,
Emrich, C.A., and Mathies, R.A. Fully Integrated PCR-Capillary Electrophoresis
Microsystem for DNA Analysis, Lab on a Chip 1:
102-107, 2001.
[Abstract]
[Full
Text in HTML] [Full
Text in PDF]
Mathies, R. A., Lagally, E.T., Kamie, T., Grover, W.H., Liu, C.N., Scherer, J.R. (2002). Capillary Array Electrophoresis Bioprocessors. Solid-State Sensor, Actuator and Microsystems Workshop, Hilton Head Island, South Carolina.