Activity-based sensing (ABS) is an emerging field that utilizes chemical reactivity, rather than conventional lock-and-key molecular recognition, to probe and manipulate biological systems. We are advancing the ABS concept and applying it to study the biology of reactive oxygen species (ROS) and their reactive sulfur and carbonyl analogs by exploiting organic and inorganic chemistries to report on these specific analytes in living cells, tissues, and animals. ABS is also being utilized to develop bioconjugation reactions for chemoproteomics, small-molecule inhibitor discovery, and antibody-drug conjugate (ADC) therapies. Finally, we are creating optogenetic tools for controlling redox signals that govern information transfer in neural circuits.


Bruemmer, K. J.*; Brewer, T. F.*; Chang, C. J. "Fluorescent probes for imaging formaldehyde in biological systems", Curr. Opin. Chem. Biol. 2017, 39, 17-23. [html] [pdf]

Brewer, T. F.; Garcia, F. J.; Onak, C. S.; Carroll, K. S.; Chang, C. J. "Chemical Approaches to Discovery and Study of Sources and Targets of Hydrogen Peroxide Redox Signaling Through NADPH Oxidase Proteins", Annu. Rev. Biochem. 2015, 84, 765-790. [html] [pdf] [Chemical & Engineering News Story]

Lin, V. S.; Chen, W.; Xian, M.; Chang, C. J. "Chemical probes for molecular imaging and detection of hydrogen sulfide and reactive sulfur species in biological systems", Chem. Soc. Rev. 2015, 44, 4596-4618. [pdf] [html]

Chan, J.; Dodani, S. C.; Chang, C. J. "Reaction-based small-molecule fluorescent probes for chemoselective bioimaging", Nature Chem. 2012, 4, 973-984. [pdf] [html]

Lippert, A. R.; Van de Bittner, G. C.; Chang, C. J. "Boronate Oxidation as a Bioorthogonal Reaction Approach for Studying the Chemistry of Hydrogen Peroxide in Living Systems", Acc. Chem. Res. 2011, 44, 793-804. [pdf] [html]


Chung, C. Y. S.; Timblin, G. A.; Saijo, K.; Chang, C. J.; "Versatile Histochemical Approach to Detection of Hydrogen Peroxide in Cells and Tissues Based on Puromycin Staining", J. Am. Chem. Soc. 2018, 140, 6109-6121. [html] [pdf]

Bruemmer, K. J.*; Walvoord, R. R.*; Brewer, T. F.; Burgos-Barragan, G.; Wit, N.; Pontel, L. B.; Patel, K. J.; Chang, C. J. "Development of a General Aza-Cope Reaction Trigger Applied to Fluorescence Imaging of Formaldehyde in Living Cells", J. Am. Chem. Soc. 2017, 139, 5338-5350. [html] [pdf]

Brewer, T. F.; Chang, C. J. "An Aza-Cope Reactivity-Based Fluorescent Probe for Imaging Formaldehyde in Living Cells", J. Am. Chem. Soc. 2015, 37, 10886–10889. [html] [pdf]

Lin, V. S.; Lippert, A. R.; Chang, C. J. "Cell-trappable Fluorescent Probes for Endogenous Hydrogen Sulfide Signaling and Imaging H2O2-Dependent H2S Production", Proc. Natl. Acad. Sci. USA 2013, 110, 7131-7135. [pdf] [html] [C&E News] [CRT Spotlight] [Faculty of 1000 Recommendation]

Michel, B. W.; Lippert, A. R.; Chang, C. J. "A Reaction-Based Fluorescent Probe for Selective Imaging of Carbon Monoxide in Living Cells Using a Palladium-Mediated Carbonylation", J. Am. Chem. Soc. 2012, 134, 15668-15671. [pdf] [html] [C&E News]

Dickinson, B. C., Peltier, J., Stone, D., Schaffer, D. V., Chang, C. J. "Nox2 redox signaling maintains essential cell populations in the brain", Nat. Chem. Biol. 2011, 7, 106-112. [pdf] [html]

Chang, M. C. Y.; Pralle, A.; Isacoff, E. Y.; Chang, C. J. "A Selective, Cell-Permeable Optical Probe for Hydrogen Peroxide in Living Cells", J. Am. Chem. Soc. 2004, 126, 15392-15393. [pdf] [C & E News]


Lin, S.; Yang, X.; Jia, S.; Weeks, A. M.; Hornsby, M.; Lee, P. S.; Nichiporuk, R. V.; Iavarone, A. T.; Wells, J. A.; Toste, F. D.; Chang, C. J. "Redox-based reagents for chemoselective methionine bioconjugation", Science 2017, 355, 597-602. [html] [pdf] [C&E News] [Berkeley Lab] [Phys. Org] [In the Pipeline] [ACS Chem. Biol.] [F1000]


Liu, W.; Truillet, C.; Flavell, R. R.; Brewer, T. F.; Evans, M. J.; Wilson, D. M.; Chang, C. J. "A reactivity-based [18F]FDG probe for in vivo formaldehyde imaging using positron emission tomography", Chem. Sci. 2016, 7, 5503-5507. [html] [pdf]

Carroll, V.; Michel, B. W.; Blecha, J.; VanBrocklin, H.; Keshari, K.; Wilson, D.; Chang, C. J. "A Boronate-Caged [18F]FLT Probe for Hydrogen Peroxide Detection Using Positron Emission Tomography", J. Am. Chem. Soc. 2014, 136, 14742-14745. [html] [pdf]

Van de Bittner, G. C.; Bertozzi, C. R.; Chang, C. J. "Strategy for Dual-Analyte Luciferin Imaging: In Vivo Bioluminescence Detection of Hydrogen Peroxide and Caspase Activity in a Murine Model of Acute Inflammation", J. Am. Chem. Soc. 2013, 135, 1783-1795. [pdf] [html]

Lippert, A. R.; Keshari, K. R.; Kurhanewicz, J.; Chang, C. J. "A Hydrogen Peroxide-Responsive Hyperpolarized 13C MRI Contrast Agent", J. Am. Chem. Soc. 2011, 133, 3776-3779. [pdf] [html]


Updated July 9 2018